| HEPES buffer | 2.3 g |
| NaNO3 | 1.5 g |
| P-IV metal solution | 1 ml |
| K2HPO4·7H2O (6 g/L) | 5 ml |
| MgSO4·7H2O (6 g/L) | 5 ml |
| Na2CO3 (4 g/L) | 5 ml |
| CaCl2·2H2O (2.5 g/L) | 10 ml |
| Na2SiO3·9H2O (4.64 g/L) | 10 ml |
| Citric acid·H2O (4.8 g/L) | 1 ml |
| Deionized water to | 200 ml |
pH 7.8
P-IV metal solution:
| Na2EDTA·2H2O | 0.75 g |
| FeCl3·6H2O | 0.097 g |
| MnCl2·4H2O | 0.041 g |
| ZnCl2 | 0.005 g |
| CoCl2·6H2O | 0.002 g |
| Na2MoO4·2H2O | 0.004 g |
| Deionized water to | 1 L |
To approximately 950 ml of dH2O, add ingredients in order listed stirring continuously. The EDTA should be fully dissoled before adding other components. Bring to 1 L with dH2O and store at 4 C.
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