Jaworski's (JM) Medium

Ca(NO3)2·4H2O (4.0 g/200 ml)1.0 ml
KH2PO4 (2.48 g/200 ml)1.0 ml
MgSO4·7H2O (10.0 g/200 ml)1.0 ml
NaHCO3 (3.18 g/200 ml)1.0 ml
EDTAFeNa (0.45 g/200 ml)1.0 ml
EDTANa2 (0.45 g/200 ml)1.0 ml
H3BO3 (0.496 g/200 ml)1.0 ml
MnCl2·4H2O (0.278 g/200 ml)1.0 ml
(NH4)6Mo7O24·4H2O (0.20 g/200 ml)1.0 ml
Cyanocobalamin (0.008 g/200 ml)1.0 ml
Thiamine HCl (0.008 g/200 ml)1.0 ml
Biotin (0.008 g/200 ml)1.0 ml
NaNO3 (16.0 g/200 ml)1.0 ml
Na2HPO4·12H2O (7.2 g/200 ml)1.0 ml
Deionized water to1.0 L
For agar, add 15.0 g/L of Bacteriological Agar (Oxoid L11)*. Autoclave at 15 psi for 15 minutes.

*Supplier: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK


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