PART 2:

NaNO3	5.00 g
K2SO4	2.00 g
NaCl	2.00 g
MgSO4·7H2O	0.40 g
CaCl2·2H2O	0.02 g
FeSO4·7H2O	0.02 g
EDTANa2	0.16 g
Micronutrient solution	10.0 ml
Vitamin solution	5.00 ml
Distilled water to	500.0 ml

Autoclave Parts 1 and 2 separately at 15 psi, allow to cool then mix aseptically.

Micronutrient solution:

ZnSO4·7H2O	0.001 g
MnSO4·7H2O	0.002 g
H3BO3	0.010 g
Na2MoO4·2H2O	0.001 g
Co(NO3)2·6H2O	0.001 g
CuSO4·5H2O	0.00005 g
FeSO4·7H2O	0.70 g
EDTANa2	0.80 g
Distilled water to	1.0 L

May be stored frozen at -20 C.

Vitamin solution:

Biotin	0.0002 g
Calcium pantothenate	0.02 g
Cyanocobalamin	0.004 g
Folic acid	0.0004 g
Inositol	1.00 g
Nicotinic acid	0.02 g
Thiamine HCl	0.10 g
Thymine	0.60 g
Distilled water to	1.0 L

May be stored frozen at -20 C.

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